nitrobenz 2 oxa 1 Search Results


pkc isoform selective  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology pkc isoform selective
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
Pkc Isoform Selective, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkc isoform selective - by Bioz Stars, 2026-11
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2-( n -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)2-deoxyglucose 2-nbdg  (Lechnologies Research Inc)
90
Lechnologies Research Inc 2-( n -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)2-deoxyglucose 2-nbdg
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
2 ( N (7 Nitrobenz 2 Oxa 1,3 Diazol 4 Yl)amino)2 Deoxyglucose 2 Nbdg, supplied by Lechnologies Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-( n -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)2-deoxyglucose 2-nbdg - by Bioz Stars, 2026-11
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2-[n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-nbdg)  (AAT Bioquest)
90
AAT Bioquest 2-[n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-nbdg)
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
2 [N (7 Nitrobenz 2 Oxa 1,3 Diazol 4 Yl)amino] 2 Deoxy D Glucose (2 Nbdg), supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-[n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-nbdg) - by Bioz Stars, 2026-11
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nbd-x (6-(n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoic acid  (AnaSpec)
90
AnaSpec nbd-x (6-(n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoic acid
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
Nbd X (6 (N (7 Nitrobenz 2 Oxa 1,3 Diazol 4 Yl)amino)hexanoic Acid, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iodoacetyl-7-nitrobenz-2-oxa-1,3-diazol ia–nbd amide  (Setareh Biotech LLC)
90
Setareh Biotech LLC iodoacetyl-7-nitrobenz-2-oxa-1,3-diazol ia–nbd amide
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
Iodoacetyl 7 Nitrobenz 2 Oxa 1,3 Diazol Ia–Nbd Amide, supplied by Setareh Biotech LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n-(7-nitrobenz-2-oxa-1, 3-diazol-4yl)-phallacidin  (Becton Dickinson)
90
Becton Dickinson n-(7-nitrobenz-2-oxa-1, 3-diazol-4yl)-phallacidin
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
N (7 Nitrobenz 2 Oxa 1, 3 Diazol 4yl) Phallacidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-nbdg)  (GlpBio Technology Inc)
90
GlpBio Technology Inc 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-nbdg)
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
2 Deoxy 2 [(7 Nitro 2,1,3 Benzoxadiazol 4 Yl)amino] D Glucose (2 Nbdg), supplied by GlpBio Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-nbdg) - by Bioz Stars, 2026-11
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2-(n-(7nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxyglucose (2-nbdg) glucose uptake assay kit  (Abnova)
90
Abnova 2-(n-(7nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxyglucose (2-nbdg) glucose uptake assay kit
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
2 (N (7nitrobenz 2 Oxa 1, 3 Diazol 4 Yl) Amino) 2 Deoxyglucose (2 Nbdg) Glucose Uptake Assay Kit, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2-(n-(7nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxyglucose (2-nbdg) glucose uptake assay kit - by Bioz Stars, 2026-11
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7-nitrobenz-2-oxa-1,3-diazole-desferrioxamine  (Squarix Biotechnology)
90
Squarix Biotechnology 7-nitrobenz-2-oxa-1,3-diazole-desferrioxamine
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
7 Nitrobenz 2 Oxa 1,3 Diazole Desferrioxamine, supplied by Squarix Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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labelled peptide derivative (fl-cwfw) containing 3-n-(7-nitrobenz-2-oxa-1,3-diazole-4-yl)-2,3-diamino-propionic acid (dap-nbd)  (BIOSYNTAN gmbh)
90
BIOSYNTAN gmbh labelled peptide derivative (fl-cwfw) containing 3-n-(7-nitrobenz-2-oxa-1,3-diazole-4-yl)-2,3-diamino-propionic acid (dap-nbd)
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
Labelled Peptide Derivative (Fl Cwfw) Containing 3 N (7 Nitrobenz 2 Oxa 1,3 Diazole 4 Yl) 2,3 Diamino Propionic Acid (Dap Nbd), supplied by BIOSYNTAN gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nbdg  (Biosynth Carbosynth)
92
Biosynth Carbosynth nbdg
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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1 2 diphytanoyl sn glycero 3 phosphocholine  (Fisher Scientific)
86
Fisher Scientific 1 2 diphytanoyl sn glycero 3 phosphocholine
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Image Search Results


FIG. 2. Transfected PKC isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC isoform. The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.

Journal: The Journal of biological chemistry

Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.

doi: 10.1074/jbc.275.3.1601

Figure Lengend Snippet: FIG. 2. Transfected PKC isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC isoform. The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.

Article Snippet: PKC isoform-selective (PKCa, sc-208; PKCd, sc-937; PKCh, sc-215; PKCe, sc-214) antibodies were from Santa Cruz Biotechnology.

Techniques: Transfection, Cell Culture, Expressing, Plasmid Preparation, Migration, Luciferase, Activity Assay

FIG. 3. Concentration dependence of PKC-dependent activa- tion of hINV gene expression. Cultured keratinocytes were trans- fected with 2 mg of pINV-241 and 0–2 mg of each PKC isoform. The total concentration of plasmid in each transfection was maintained constant at 4 mg by addition of empty expression vector. After 24 h extracts were prepared and assayed for luciferase activity.

Journal: The Journal of biological chemistry

Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.

doi: 10.1074/jbc.275.3.1601

Figure Lengend Snippet: FIG. 3. Concentration dependence of PKC-dependent activa- tion of hINV gene expression. Cultured keratinocytes were trans- fected with 2 mg of pINV-241 and 0–2 mg of each PKC isoform. The total concentration of plasmid in each transfection was maintained constant at 4 mg by addition of empty expression vector. After 24 h extracts were prepared and assayed for luciferase activity.

Article Snippet: PKC isoform-selective (PKCa, sc-208; PKCd, sc-937; PKCh, sc-215; PKCe, sc-214) antibodies were from Santa Cruz Biotechnology.

Techniques: Concentration Assay, Gene Expression, Cell Culture, Plasmid Preparation, Transfection, Expressing, Luciferase, Activity Assay

FIG. 8. Regulation of PKC isoform level by TPA. Keratinocytes were treated with 50 ng/ml TPA for 24 h followed by preparation of nuclear extracts. Equivalent quantities of extract, layered based on protein concentration, were electrophoresed on an 8% acrylamide gel and transferred to nitrocellulose for detection using PKCe-, -d-, and -h-specific antibodies. Binding of the primary antibody was detected by incubation with an appropriate secondary antibody, and binding was visualized using ECL technology.

Journal: The Journal of biological chemistry

Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.

doi: 10.1074/jbc.275.3.1601

Figure Lengend Snippet: FIG. 8. Regulation of PKC isoform level by TPA. Keratinocytes were treated with 50 ng/ml TPA for 24 h followed by preparation of nuclear extracts. Equivalent quantities of extract, layered based on protein concentration, were electrophoresed on an 8% acrylamide gel and transferred to nitrocellulose for detection using PKCe-, -d-, and -h-specific antibodies. Binding of the primary antibody was detected by incubation with an appropriate secondary antibody, and binding was visualized using ECL technology.

Article Snippet: PKC isoform-selective (PKCa, sc-208; PKCd, sc-937; PKCh, sc-215; PKCe, sc-214) antibodies were from Santa Cruz Biotechnology.

Techniques: Protein Concentration, Acrylamide Gel Assay, Binding Assay, Incubation